Antibacterial Activity Test of Ethyl Ascetic Fraction Ethanol Extract Faloak Tree Bark (sterculia sp.) on Salmonella Thyposa Bacteria
The bark of the faloak (Sterculia sp.) Tree utilized by the people of Timor Island is limited as a source of medicine. The efficacious substances in faloak bark which are known to have antibacterial properties are flavonoids, alkaloids and saponins. The purpose of this study was to measure the inhibitory power of ethyl acetate fraction of ethanol extract of faloak tree bark (Sterculia sp.) Against the growth of Salmonella thyposa bacteria. Ethyl acetate fraction was obtained from the bark of faloak (Sterculia sp.) Tree which was macerated with 70% ethanol so that the ethanol extract from the bark of faloak tree (Sterculia sp.) Was obtained. The extract was fractionated using a liquid-liquid partition with a mixture of ethanol-water (2: 3) and ethyl acetate. The results of the fraction were identified qualitatively, then antibacterial activity was tested using diffusion method with antibacterial effectiveness parameters based on the diameter of the inhibition zone around the cylinder. Antibacterial activity test carried out on ethyl acetate fraction obtained from fractionation of ethanol extract of faloak (Sterculia sp.) Tree bark was carried out with 3 concentration treatments namely 25%, 50% and 75% and 3 replications for each treatment. The measurement of inhibition zone diameter showed that ethyl acetate fraction of ethanol extract of the bark of faloak (Sterculia sp.) At a concentration of 25% b / v, 50% b / v did not show any antibacterial activity, as seen from the absence of the resulting inhibition zone while at a concentration of 75% b / v showed antibacterial activity against Salmonella typhoid with an area of 15 mm inhibition zone. The conclusion was that ethyl acetate fraction of ethanol extract from the bark of faloak tree (Sterculia sp.) Had antibacterial activity against Salmonella thyposa bacteria at a concentration of 75% b / v.