Antioxidant Activity Of Purified Leaf Extract Of Moringa (Moringa Oleifera, Lam)
Background: Moringa (Moringa Oleifera, Lam) which is rich in macronutrients and micronutrients has also been known to have antioxidant activity. Several studies have shown that both crude extract and moringa leaf infusion have activities as antioxidants with varying strengths. The efforts to increase the levels of active compound higher than crude extracts is by purifying or removing ballast substances such as chlorophyll which is widely found in Moringa leaves. Objective: To determine the antioxidant activity of Moringa purified extract. Method: Moringa leaves were collected from Oesapa, Kupang, East Nusa Tenggara and extracted with ethanol by using maceration for the first time (crude extract) and purified by liquid-liquid partitioning method using dichloromethane and methanol-water mixture to removed chlorophyll (Purified extract). The purified extract in various concentrations (250 to 500 mg/mL) and crude extract (50 to 300 mg/mL) were screened for antioxidant activity by free radical scavenging activity (DPPH) method. Moreover, Qualitative analysis for determining the presence of tannins and polyphenol in the purified extract was performed using Ferric Chloride 1% and flavonoid presence using TLC profiling with the standard quercetin. The statistical analysis and linear regression analysis were performed using GraphPad Instat, software, version 7.0. The IC50 values were calculated and compared by paired t-tests. p<0.05 was considered significant. Result: The qualitative analysis of the purified extract revealed the presence of tannin and polyphenol and Chromatogram profiling revealed the presence of flavonoid. The radical scavenging activity expressed as a half-maximal inhibitory concentration (IC50) of purified extract (154±18,04 µg/mL) was found significantly higher to crude extract (408, 0±30,16 µg/mL). A purified extract of Moringa Leaves has radical scavenging activity and was found significantly higher to crude extract.